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anti his pe  (R&D Systems)


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    Structured Review

    R&D Systems anti his pe
    Anti His Pe, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+his+pe/His+Tag+PE-conjugated+Antibody/us12612461-459-79-80
    Average 93 stars, based on 27 article reviews
    anti his pe - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Anti-CD19 CAR T cells potently redirected to kill solid tumor cells.
    Article Snippet: .. The cells were resuspended in 100 μl total volume and stained with 5 μl anti-His-PE (#IC050P, R&D Systems, Minneapolis, MN) or anti-CD19 antibody FMC63-PE (#MAB1794, MilliporeSigma, Burlington, MA) for 30 minutes at 4 ̊C, washed and resuspended using PBS, fixed with 1% paraformadehyde in PBS, and analyzed on the Accuri Flow Cytometer (BD Biosciences). ..

    Article Title: Anti-CD19 CAR T cells potently redirected to kill solid tumor cells
    Article Snippet: .. The cells were resuspended in 100 μl total volume and stained with 5 μl anti-His-PE (#IC050P, R&D Systems, Minneapolis, MN) or anti-CD19 antibody FMC63-PE (#MAB1794, MilliporeSigma, Burlington, MA) for 30 minutes at 40C, washed and resuspended using PBS, fixed with 1% paraformadehyde in PBS, and analyzed on the Accuri Flow Cytometer (BD Biosciences). ..

    Article Title: Anti-CD19 CAR T cells potently redirected to kill solid tumor cells
    Article Snippet: .. The cells were resuspended in 100 μl total volume and stained with 5 μl anti-His-PE (#IC050P, R&D Systems, Minneapolis, MN) or anti-CD19 antibody FMC63-PE (#MAB1794, MilliporeSigma, Burlington, MA) for 30 minutes at 40C, washed and resuspended using PBS, fixed with 1% paraformadehyde in PBS, and analyzed on the Accuri Flow Cytometer (BD Biosciences). ..

    Flow Cytometry:

    Article Title: Anti-CD19 CAR T cells potently redirected to kill solid tumor cells.
    Article Snippet: .. The cells were resuspended in 100 μl total volume and stained with 5 μl anti-His-PE (#IC050P, R&D Systems, Minneapolis, MN) or anti-CD19 antibody FMC63-PE (#MAB1794, MilliporeSigma, Burlington, MA) for 30 minutes at 4 ̊C, washed and resuspended using PBS, fixed with 1% paraformadehyde in PBS, and analyzed on the Accuri Flow Cytometer (BD Biosciences). ..

    Article Title: Anti-CD19 CAR T cells potently redirected to kill solid tumor cells
    Article Snippet: .. The cells were resuspended in 100 μl total volume and stained with 5 μl anti-His-PE (#IC050P, R&D Systems, Minneapolis, MN) or anti-CD19 antibody FMC63-PE (#MAB1794, MilliporeSigma, Burlington, MA) for 30 minutes at 40C, washed and resuspended using PBS, fixed with 1% paraformadehyde in PBS, and analyzed on the Accuri Flow Cytometer (BD Biosciences). ..

    Article Title: Anti-CD19 CAR T cells potently redirected to kill solid tumor cells
    Article Snippet: .. The cells were resuspended in 100 μl total volume and stained with 5 μl anti-His-PE (#IC050P, R&D Systems, Minneapolis, MN) or anti-CD19 antibody FMC63-PE (#MAB1794, MilliporeSigma, Burlington, MA) for 30 minutes at 40C, washed and resuspended using PBS, fixed with 1% paraformadehyde in PBS, and analyzed on the Accuri Flow Cytometer (BD Biosciences). ..

    Blocking Assay:

    Article Title: Single domain antibodies against CLL-1
    Article Snippet: .. Briefly, the method included: U937 cells (2.5×10{circumflex over ( )}5) were Fc blocked (human Fc block, BD Cat. #BDB564220) for 10 min at room temperature, then washed once with FACS buffer (PBS+1% BSA+0.1% Sodium Azide) by spinning at 500G for 2 min; VHH clones (3 μg/ml as final conc) in FACS buffer were added to the cell pellets and incubated for 30 min at 4° C., then washed twice with FACS buffer by spinning at 500G for 2 min; anti-His-PE (R&D Systems, Cat. #IC050P) was added to cells; the mixture was incubated for 30 min at 4° C. and washed twice with FACS buffer by spinning at 500G for 2 min; 1% PFA (Paraformaldehyde) in PBS was added to cell pellet to fix the cells, followed by FACS analysis. ..

    FACS:

    Article Title: Single domain antibodies against CLL-1
    Article Snippet: .. Briefly, the method included: U937 cells (2.5×10{circumflex over ( )}5) were Fc blocked (human Fc block, BD Cat. #BDB564220) for 10 min at room temperature, then washed once with FACS buffer (PBS+1% BSA+0.1% Sodium Azide) by spinning at 500G for 2 min; VHH clones (3 μg/ml as final conc) in FACS buffer were added to the cell pellets and incubated for 30 min at 4° C., then washed twice with FACS buffer by spinning at 500G for 2 min; anti-His-PE (R&D Systems, Cat. #IC050P) was added to cells; the mixture was incubated for 30 min at 4° C. and washed twice with FACS buffer by spinning at 500G for 2 min; 1% PFA (Paraformaldehyde) in PBS was added to cell pellet to fix the cells, followed by FACS analysis. ..

    Clone Assay:

    Article Title: Single domain antibodies against CLL-1
    Article Snippet: .. Briefly, the method included: U937 cells (2.5×10{circumflex over ( )}5) were Fc blocked (human Fc block, BD Cat. #BDB564220) for 10 min at room temperature, then washed once with FACS buffer (PBS+1% BSA+0.1% Sodium Azide) by spinning at 500G for 2 min; VHH clones (3 μg/ml as final conc) in FACS buffer were added to the cell pellets and incubated for 30 min at 4° C., then washed twice with FACS buffer by spinning at 500G for 2 min; anti-His-PE (R&D Systems, Cat. #IC050P) was added to cells; the mixture was incubated for 30 min at 4° C. and washed twice with FACS buffer by spinning at 500G for 2 min; 1% PFA (Paraformaldehyde) in PBS was added to cell pellet to fix the cells, followed by FACS analysis. ..

    Incubation:

    Article Title: Single domain antibodies against CLL-1
    Article Snippet: .. Briefly, the method included: U937 cells (2.5×10{circumflex over ( )}5) were Fc blocked (human Fc block, BD Cat. #BDB564220) for 10 min at room temperature, then washed once with FACS buffer (PBS+1% BSA+0.1% Sodium Azide) by spinning at 500G for 2 min; VHH clones (3 μg/ml as final conc) in FACS buffer were added to the cell pellets and incubated for 30 min at 4° C., then washed twice with FACS buffer by spinning at 500G for 2 min; anti-His-PE (R&D Systems, Cat. #IC050P) was added to cells; the mixture was incubated for 30 min at 4° C. and washed twice with FACS buffer by spinning at 500G for 2 min; 1% PFA (Paraformaldehyde) in PBS was added to cell pellet to fix the cells, followed by FACS analysis. ..



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    Image Search Results


    Trifunctional fusion proteins enhance cytotoxic potential differently. Fusion proteins were incubated (A) untargeted or (B) targeted to B16-FAP cells (preincubated with the indicated concentration and then washed to remove unbound fusion protein) with PBMCs in presence of suboptimal concentration of CytoStim™. After 4 days, cells were fixed, permeabilized and intracellularly stained with anti-CD3, anti-CD8 and anti-granzyme B antibodies, followed by flow cytometry analysis. Graphics show mean ± SD, n=3, independent experiments, using PBMCs from the same donor and fusion proteins from batches #2, with blockshift correction, One-way ANOVA, Tukey’s post-test **p<0.01; ***p<0.001; ****p<0.0001.

    Journal: Frontiers in Immunology

    Article Title: Trifunctional antibody fusion proteins for targeted co-delivery of 4-1BBL with IL-15 or IL-21 to enhance antitumor immune responses

    doi: 10.3389/fimmu.2026.1903525

    Figure Lengend Snippet: Trifunctional fusion proteins enhance cytotoxic potential differently. Fusion proteins were incubated (A) untargeted or (B) targeted to B16-FAP cells (preincubated with the indicated concentration and then washed to remove unbound fusion protein) with PBMCs in presence of suboptimal concentration of CytoStim™. After 4 days, cells were fixed, permeabilized and intracellularly stained with anti-CD3, anti-CD8 and anti-granzyme B antibodies, followed by flow cytometry analysis. Graphics show mean ± SD, n=3, independent experiments, using PBMCs from the same donor and fusion proteins from batches #2, with blockshift correction, One-way ANOVA, Tukey’s post-test **p<0.01; ***p<0.001; ****p<0.0001.

    Article Snippet: Antibodies and other reagents were purchased from BioLegend (anti-CD3 PE, 317308; anti-CD8 Pacific Blue, 344718; anti-CD56 APC, 362504; anti-CD69 FITC, 310904; anti-CD56 PacificBlue, 362519; FoxP3 Fix/Perm buffer,421401; FoxP3 Perm buffer, 421402), Miltenyi Biotech (anti-His-PE, 130-120-718; anti-CD8 PEVio770.

    Techniques: Incubation, Concentration Assay, Staining, Flow Cytometry, Analysis